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ISSN 1449-2288 |
Int J Biol Sci 2008; 4:245 ©Ivyspring International Publisher Letter To The Editor In reply to Zou et al. “New amyloid plaques or a game of hide-and-seek?” Massachusetts General Hospital, MassGeneral Institute for Neurodegeneration, Charlestown MA 02129, USA How
to cite this article:
Hyman B. In reply to Zou et al. “New amyloid plaques or a game of hide-and-seek?”. Int J Biol Sci 2008; 4:245. Available from http://www.biolsci.org/v04p0245.htm Reply to the article: Zou K, Maeda T, Michikawa M, Komano H. New amyloid plaques or a game of hide-and-seek? Int J Biol Sci 2008; 4:200-201. We appreciate, but disagree with, Zou et al's comments regarding potential technical issues in the in vivo imaging. Our control experiments, including explicit criteria for inclusion and exclusion of new plaques, the presence of other plaques in the field that are observed by the dyes, the use of different freely diffusible well characterized dyes (both topical application and intraperitoneal application for a dye that that we have previously formally demonstrated to readily cross the blood brain barrier) and concurrent imaging of neurites and blood vessels to ensure quality of imaging per se all, in our opinion, argue against their concerns. Correspondence to: Bradley Hyman MD PhD, John B Penney Jr Professor of Neurology Harvard Medical School, Massachusetts General Hospital, MassGeneral Institute for Neurodegeneration Rm 2009, 114 16th Street, Charlestown MA 02129. Author contact✉ Correspondence to: Bradley Hyman MD PhD, John B Penney Jr Professor of Neurology Harvard Medical School, Massachusetts General Hospital, MassGeneral Institute for Neurodegeneration Rm 2009, 114 16th Street, Charlestown MA 02129. Reply to the article: Zou K, Maeda T, Michikawa M, Komano H. New amyloid plaques or a game of hide-and-seek? Int J Biol Sci 2008; 4:200-201. We appreciate, but disagree with, Zou et al's comments regarding potential technical issues in the in vivo imaging. Our control experiments, including explicit criteria for inclusion and exclusion of new plaques, the presence of other plaques in the field that are observed by the dyes, the use of different freely diffusible well characterized dyes (both topical application and intraperitoneal application for a dye that that we have previously formally demonstrated to readily cross the blood brain barrier) and concurrent imaging of neurites and blood vessels to ensure quality of imaging per se all, in our opinion, argue against their concerns. Published 2008-8-8 |