Int J Biol Sci 2011; 7(3):253-260. doi:10.7150/ijbs.7.253 This issue Cite

Research Paper

Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System

Hiroshi Umakoshi, Tomoyuki Tanabe, Keishi Suga, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Osaka, Japan

Citation:
Umakoshi H, Tanabe T, Suga K, Bui HT, Shimanouchi T, Kuboi R. Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System. Int J Biol Sci 2011; 7(3):253-260. doi:10.7150/ijbs.7.253. https://www.ijbs.com/v07p0253.htm
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Abstract

Oxidative stress can affect in vitro GFP expression through its control of the gene silencing effect of the liposome prepared by 1,2-dioleoyl-3-trimethyl-ammonium propane (DOTAP). The gene silencing effect of cationic DOTAP liposome in in vitro GFP expression, especially focusing on its translation process, and the effects of oxidative stress on its silencing effect were investigated. GFP expression, initiated by mRNA, was found to be thoroughly inhibited in the presence of DOTAP liposome at concentration of more than 2.5 mM, though its inhibitory effect was reduced in the presence of hydrogen peroxide. The analyses of (i) the interaction of mRNA with DOTAP, (ii) the chemical structure of DOTAP, and (iii) the membrane fluidity of DOTAP liposome imply the possible role of gene expression by the liposome membrane and stress conditions.

Keywords: Oxidative stress, GFP expression, gene silencing, liposome


Citation styles

APA
Umakoshi, H., Tanabe, T., Suga, K., Bui, H.T., Shimanouchi, T., Kuboi, R. (2011). Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System. International Journal of Biological Sciences, 7(3), 253-260. https://doi.org/10.7150/ijbs.7.253.

ACS
Umakoshi, H.; Tanabe, T.; Suga, K.; Bui, H.T.; Shimanouchi, T.; Kuboi, R. Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System. Int. J. Biol. Sci. 2011, 7 (3), 253-260. DOI: 10.7150/ijbs.7.253.

NLM
Umakoshi H, Tanabe T, Suga K, Bui HT, Shimanouchi T, Kuboi R. Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System. Int J Biol Sci 2011; 7(3):253-260. doi:10.7150/ijbs.7.253. https://www.ijbs.com/v07p0253.htm

CSE
Umakoshi H, Tanabe T, Suga K, Bui HT, Shimanouchi T, Kuboi R. 2011. Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System. Int J Biol Sci. 7(3):253-260.

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